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Anti-APPBP1 Picoband Antibody

     
  • WB - Anti-APPBP1 Picoband Antibody ABO12030
    Anti- APPBP1 Picoband antibody, ABO12030, Western blottingAll lanes: Anti APPBP1 (ABO12030) at 0.5ug/mlWB: Recombinant Human APPBP1 Protein 0.5ngPredicted bind size: 42KDObserved bind size: 42KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q13564
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for NEDD8-activating enzyme E1 regulatory subunit(NAE1) detection. Tested with WB in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 8883
Other Names NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding protein 1, 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1, APPBP1
Calculated MW 60246 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Cell membrane . Colocalizes with APP in lipid rafts.
Tissue Specificity Ubiquitous in fetal tissues. Expressed throughout the adult brain. .
Protein Name NEDD8-activating enzyme E1 regulatory subunit
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human APPBP1 recombinant protein (Position: E181-L534). Human APPBP1 shares 98% amino acid (aa) sequence identity with mouse APPBP1.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name NAE1
Synonyms APPBP1
Function Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development.
Cellular Location Cell membrane. Note=Colocalizes with APP in lipid rafts
Tissue Location Ubiquitous in fetal tissues. Expressed throughout the adult brain.
Research Areas
Citations (0)
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Background

NAE1 is also known as APPBP1. The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.

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$ 370.00
Cat# ABO12030
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