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Anti-MGMT Picoband Antibody

     
  • WB - Anti-MGMT Picoband Antibody ABO11957
    Anti- MGMT Picoband antibody, ABO11957, Western blottingAll lanes: Anti MGMT (ABO11957) at 0.5ug/mlWB: Recombinant Human MGMT Protein 0.5ngPredicted bind size: 21KDObserved bind size: 21KD
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  • WB - Anti-MGMT Picoband Antibody ABO11957
    Anti- MGMT Picoband antibody, ABO11957, Western blottingAll lanes: Anti MGMT (ABO11957) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 21KDObserved bind size: 21KD
    detail
  • IHC - Anti-MGMT Picoband Antibody ABO11957
    Anti- MGMT Picoband antibody, ABO11957, IHC(P)IHC(P): Human Placenta Tissue
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC
Primary Accession P16455
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Methylated-DNA--protein-cysteine methyltransferase(MGMT) detection. Tested with WB, IHC-P, ICC in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 4255
Other Names Methylated-DNA--protein-cysteine methyltransferase, 2.1.1.63, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT
Calculated MW 21646 MW KDa
Application Details Immunocytochemistry , 0.5-1 µg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat
Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Nucleus.
Protein Name Methylated-DNA--protein-cysteine methyltransferase
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human MGMT recombinant protein (Position: D2-N207). Human MGMT shares 70% and 69% amino acid (aa) sequence identity with mouse and rat MGMT, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name MGMT
Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Cellular Location Nucleus.
Citations (0)
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Background

O6-alkylguanine DNA alkyltransferase (also known as AGT, MGMT or AGAT) is a protein that in humans is encoded by the O6-methylguanine DNA methyltransferase (MGMT) gene. MGMT is mapped to 10q26.3. This gene is crucial for genome stability. It repairs the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine and prevents mismatch and errors during DNA replication and transcription. It has been found that methylation of the MGMT promoter in the tumor was associated with longer survival.

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$ 370.00
Cat# ABO11957
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