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Anti-Mitofusin 1 Picoband Antibody

     
  • WB - Anti-Mitofusin 1 Picoband Antibody ABO11955
    Anti- Mitofusin 1 Picoband antibody, ABO11955, Western blottingAll lanes: Anti Mitofusin 1 (ABO11955) at 0.5ug/mlWB: Recombinant Human Mitofusin 1 Protein 0.5ngPredicted bind size: 45KDObserved bind size: 45KD
    detail
  • WB - Anti-Mitofusin 1 Picoband Antibody ABO11955
    Anti- Mitofusin 1 Picoband antibody, ABO11955, Western blottingAll lanes: Anti Mitofusin 1 (ABO11955) at 0.5ug/mlLane 1: Rat Cardiac Muscle Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Mouset Cardiac Muscle Tissue Lysate at 50ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugPredicted bind size: 84KDObserved bind size: 84KD
    detail
Anti- Mitofusin 1 Picoband antibody, ABO11955, Western blottingAll lanes: Anti Mitofusin 1 (ABO11955) at 0.5ug/mlWB: Recombinant Human Mitofusin 1 Protein 0.5ngPredicted bind size: 45KDObserved bind size: 45KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q8IWA4
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Mitofusin-1(MFN1) detection. Tested with WB in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 55669
Other Names Mitofusin-1, 3.6.5.-, Fzo homolog, Transmembrane GTPase MFN1, MFN1
Calculated MW 84100 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Mitochondrion outer membrane ; Multi- pass membrane protein .
Tissue Specificity Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers. .
Protein Name Mitofusin-1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1 (23-49aa DQLLEFVTEGSHFVEATYKNPELDRIA), different from the related mouse and rat sequences by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
Protein Information
Name MFN1
Function Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12475957, PubMed:12759376, PubMed:27920125, PubMed:28114303). Membrane clustering requires GTPase activity (PubMed:27920125). It may involve a major rearrangement of the coiled coil domains (PubMed:27920125, PubMed:28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12475957, PubMed:12759376). Overexpression induces the formation of mitochondrial networks (in vitro) (PubMed:12759376). Has low GTPase activity (PubMed:27920125, PubMed:28114303).
Cellular Location Mitochondrion outer membrane; Multi-pass membrane protein
Tissue Location Detected in kidney and heart (at protein level) (PubMed:12759376). Ubiquitous (PubMed:11950885, PubMed:12759376) Expressed at slightly higher level in kidney and heart (PubMed:12759376). Isoform 2 may be overexpressed in some tumors, such as lung cancers (PubMed:11751411).
Research Areas
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$ 370.00
Cat# ABO11955
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