Anti-IL-16 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, ICC, E |
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Primary Accession | Q14005 |
Host | Rabbit |
Reactivity | Human |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Pro-interleukin-16(IL16) detection. Tested with WB, IHC-P, ICC, ELISA in Human. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 3603 |
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Other Names | Pro-interleukin-16, Interleukin-16, IL-16, Lymphocyte chemoattractant factor, LCF, IL16 |
Calculated MW | 141752 MW KDa |
Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat Immunocytochemistry , 0.5-1 µg/ml, Human, - Western blot, 0.1-0.5 µg/ml, Human ELISA, 0.1-0.5 µg/ml, Human |
Subcellular Localization | Interleukin-16: Secreted. |
Tissue Specificity | Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation. |
Protein Name | Pro-interleukin-16 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen | E.coli-derived human IL-16 recombinant protein (Position: S1212-S1332). Human IL-16 shares 86% amino acid (aa) sequence identity with mouse IL-16. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Contains 4 PDZ (DHR) domains. |
Name | IL16 |
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Function | Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA- binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. |
Cellular Location | [Interleukin-16]: Secreted. [Isoform 3]: Cytoplasm. Nucleus. |
Tissue Location | [Isoform 3]: Expressed in hemopoietic tissues, such as resting T-cells, but undetectable during active T-cell proliferation |
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Provided below are standard protocols that you may find useful for product applications.
Background
Interleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. It is mapped to 15q25.1. IL-16 was originally described as a factor that could attract activated T cells in humans. It was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16 stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.
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