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Anti-HNF1 Picoband Antibody

     
  • WB - Anti-HNF1 Picoband Antibody ABO11905
    Anti- HNF1 antibody, ABO11905, Western blottingAll lanes: Anti HNF1 (ABO11905) at 0.5ug/mlWB: Recombinant Human HNF1 Protein 0.5ngPredicted bind size: 45KDObserved bind size: 45KD
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  • WB - Anti-HNF1 Picoband Antibody ABO11905
    Anti- HNF1 antibody, ABO11905, Western blottingAll lanes: Anti HNF1 (ABO11905) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: RH35 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 67KDObserved bind size: 67KD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P20823
Host Rabbit
Reactivity Human, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Hepatocyte nuclear factor 1-alpha(HNF1A) detection. Tested with WB in Human;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 6927
Other Names Hepatocyte nuclear factor 1-alpha, HNF-1-alpha, HNF-1A, Liver-specific transcription factor LF-B1, LFB1, Transcription factor 1, TCF-1, HNF1A, TCF1
Calculated MW 67356 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human, Rat
Subcellular Localization Nucleus .
Tissue Specificity Liver.
Protein Name Hepatocyte nuclear factor 1-alpha
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human HNF1 recombinant protein (Position: A431-Q631). Human HNF1 shares 93% and 92% amino acid (aa) sequences identity with mouse and rat HNF1, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the HNF1 homeobox family.
Protein Information
Name HNF1A
Synonyms TCF1
Function Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'- GTTAATNATTAAC-3' (PubMed:10966642, PubMed:12453420). Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:10966642, ECO:0000269|PubMed:38018242}
Tissue Location Liver.
Citations (0)
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Background

HNF1 homeobox A (hepatocyte nuclear factor 1 homeobox A), also known as HNF1A or TCF1, is a human gene. It is mapped to chromosome 12q24.3. The protein encoded by this gene is a transcription factor that is highly expressed in the liver and is involved in the regulation of the expression of several liver-specific genes. HNF1A can function as a dimer. What’s more, deletion of the HNF1A activation domains or interruption of the HNF1-binding sites in the LCT promoter resulted in complete loss of transcriptional activity.

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$ 370.00
Cat# ABO11905
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