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Anti-EDA Picoband Antibody

     
  • WB - Anti-EDA Picoband Antibody ABO11882
    Anti- EDA antibody, ABO11882, Western blottingAll lanes: Anti EDA (ABO11882) at 0.5ug/mlWB: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 41KDObserved bind size: 41KD
    detail
  • WB - Anti-EDA Picoband Antibody ABO11882
    Anti- EDA antibody, ABO11882, Western blottingAll lanes: Anti EDA (ABO11882) at 0.5ug/mlWB : Recombinant Human EDA Protein 0.5ngPredicted bind size: 43KDObserved bind size: 43KD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q92838
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Ectodysplasin-A(EDA) detection. Tested with WB in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 1896
Other Names Ectodysplasin-A, Ectodermal dysplasia protein, EDA protein, Ectodysplasin-A, membrane form, Ectodysplasin-A, secreted form, EDA, ED1, EDA2
Calculated MW 41294 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Cell membrane; Single-pass type II membrane protein.
Tissue Specificity Not abundant; expressed in specific cell types of ectodermal (but not mesodermal) origin of keratinocytes, hair follicles, sweat glands. Also in adult heart, liver, muscle, pancreas, prostate, fetal liver, uterus, small intestine and umbilical chord. .
Protein Name Ectodysplasin-A
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human EDA recombinant protein (Position: A30-S391). Human EDA shares 95% amino acid (aa) sequence identity with mouse EDA.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the tumor necrosis factor family.
Protein Information
Name EDA
Synonyms ED1, EDA2
Function Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R (PubMed:11039935, PubMed:27144394, PubMed:34582123, PubMed:8696334). May also play a role in cell adhesion (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:O54693}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:O54693}
Tissue Location Not abundant; expressed in specific cell types of ectodermal (but not mesodermal) origin of keratinocytes, hair follicles, sweat glands. Also in adult heart, liver, muscle, pancreas, prostate, fetal liver, uterus, small intestine and umbilical chord {ECO:0000269|Ref.6}
Research Areas
Citations (0)
citation

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Background

Ectodysplasin-A is a protein that in humans is encoded by the EDA gene. It is mapped to Xq13.1. The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia.

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$ 370.00
Cat# ABO11882
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