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Anti-GST3/GST Pi Picoband Antibody

     
  • WB - Anti-GST3/GST Pi Picoband Antibody ABO11876
    Anti- GST3/GST pi Picoband antibody, ABO11876, Western blottingAll lanes: Anti GST3/GST pi(ABO11876) at 0.5ug/mlWB: Recombinant Human GST3 Protein 0.5ngPredicted bind size: 32KDObserved bind size: 32KD
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  • WB - Anti-GST3/GST Pi Picoband Antibody ABO11876
    Anti- GST3/GST pi Picoband antibody, ABO11876, Western blottingAll lanes: Anti GST3/GST pi (ABO11876) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugLane 4: Hela Whole Cell Lysate at 40ugLane 5: HT1080 Whole Cell Lysate at 40ugLane 6: MCF-7 Whole Cell Lysate at 40ugLane 7: SW620 Whole Cell Lysate at 40ugPredicted bind size: 23KD Observed bind size: 23KD
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  • IHC - Anti-GST3/GST Pi Picoband Antibody ABO11876
    Anti- GST3/GST pi Picoband antibody, ABO11876, IHC(P)IHC(P): Rat Lung Tissue
    detail
  • IHC - Anti-GST3/GST Pi Picoband Antibody ABO11876
    Anti- GST3/GST pi Picoband antibody, ABO11876, IHC(P)IHC(P): Human Lung Cancer Tissue
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P09211
Host Rabbit
Reactivity Human, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Glutathione S-transferase P(GSTP1) detection. Tested with WB, IHC-P in Human;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 2950
Other Names Glutathione S-transferase P, 2.5.1.18, GST class-pi, GSTP1-1, GSTP1, FAEES3, GST3
Calculated MW 23356 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat
Subcellular Localization Cytoplasm . Mitochondrion . Nucleus . The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization.
Protein Name Glutathione S-transferase P
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human GST3 recombinant protein (Position: P2-Q210). Human GST3 shares 85% and 86% amino acid (aa) sequences identity with mouse and rat GST3, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the GST superfamily. Pi family.
Protein Information
Name GSTP1 (HGNC:4638)
Synonyms FAEES3, GST3
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
Cellular Location Cytoplasm. Mitochondrion. Nucleus. Note=The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization
Research Areas
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Background

Glutathione S-transferases pi (GSTP1), also known as GST3, is an enzyme that in humans is encoded by the GSTP1 gene. This gene is mapped to 11q13.2. GSTP1 has 7 exons and 6 introns contained within approximately 2.8 kilobases. GSTP1 belongs to Glutathione S-transferases (GSTs) which are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 presents in all tissues and cells, with the exception of red cells, in which only erythrocyte GST(GSTe) is observed. What's more, GSTP1 is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

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$ 370.00
Cat# ABO11876
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