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Anti-DHFR Picoband Antibody

     
  • WB - Anti-DHFR Picoband Antibody ABO11868
    Anti- DHFR Picoband antibody, ABO11868, Western blottingAll lanes: Anti DHFR (ABO11868) at 0.5ug/mlWB: Recombinant Human DHFR Protein 0.5ngPredicted bind size: 32KDObserved bind size: 32KD
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  • WB - Anti-DHFR Picoband Antibody ABO11868
    Anti- DHFR Picoband antibody, ABO11868, Western blottingAll lanes: Anti DHFR (ABO11868) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugLane 3: HepG2 Whole Cell Lysate at 40ugLane 4: Jurkat Whole Cell Lysate at 40ugPredicted bind size: 21KDObserved bind size: 21KD
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  • IHC - Anti-DHFR Picoband Antibody ABO11868
    Anti- DHFR Picoband antibody, ABO11868,IHC(P)IHC(P): Mouse Brain Tissue
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  • IHC - Anti-DHFR Picoband Antibody ABO11868
    Anti- DHFR Picoband antibody, ABO11868,IHC(P)IHC(P): Rat Brain Tissue
    detail
  • IHC - Anti-DHFR Picoband Antibody ABO11868
    Anti- DHFR Picoband antibody, ABO11868,IHC(P)IHC(P): Human Lung Cancer Tissue
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P00374
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Dihydrofolate reductase(DHFR) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 1719
Other Names Dihydrofolate reductase, 1.5.1.3, DHFR
Calculated MW 21453 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Tissue Specificity Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain. .
Protein Name Dihydrofolate reductase
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the dihydrofolate reductase family.
Protein Information
Name DHFR
Function Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Cellular Location Mitochondrion {ECO:0000250|UniProtKB:P00375}. Cytoplasm {ECO:0000250|UniProtKB:P00375}
Tissue Location Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood Expression is higher in the adult brain than in the fetal brain
Research Areas
Citations (0)
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Background

Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. In humans, the DHFR enzyme is encoded by the DHFR gene. It is found in the q11→q22 region of chromosome 5. What's more, DHFR belongs to the dihydrofolate reductase family, and it converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. DHFR is the key enzyme in folate metabolism. In addition, DHFR catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

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$ 370.00
Cat# ABO11868
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