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Anti-AFP Picoband Antibody

     
  • WB - Anti-AFP Picoband Antibody ABO11799
    Anti-AFP Picoband antibody, ABO11799-1.jpgAll lanes: Anti AFP (ABO11799) at 0.5ug/mlWB: Human Recombinant AFP Protein 0.5ngPredicted bind size: 36KDObserved bind size: 36KD
    detail
  • WB - Anti-AFP Picoband Antibody ABO11799
    Anti-AFP Picoband antibody, ABO11799-2.jpgAll lanes: Anti AFP (ABO11799) at 0.5ug/mlWB: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 69KDObserved bind size: 69KD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P02771
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Alpha-fetoprotein(AFP) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 174
Other Names Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP
Calculated MW 68678 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Secreted.
Tissue Specificity Plasma. Synthesized by the fetal liver and yolk sac.
Protein Name Alpha-fetoprotein
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the ALB/AFP/VDB family.
Protein Information
Name AFP
Synonyms HPAFP
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Cellular Location Secreted.
Tissue Location Plasma. Synthesized by the fetal liver and yolk sac
Research Areas
Citations (0)
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Background

AFP, also called Alpha-fetoprotein; alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a diagnostic marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a diagnostic marker in biliary atresia, hepatitis, and yolk sac derived tumours.

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$ 370.00
Cat# ABO11799
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