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Anti-PC4 Picoband Antibody

     
  • WB - Anti-PC4 Picoband Antibody ABO11716
    Western blot analysis of PC4 expression in rat liver extract (lane 1), HELA whole cell lysates (lane 2) and U2OS whole cell lysates (lane 3). PC4 at 19KD was detected using rabbit anti- PC4 Antigen Affinity purified polyclonal antibody (Catalog # ABO11716) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
    detail
  • IHC - Anti-PC4 Picoband Antibody ABO11716
    PC4 was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti- PC4 Antigen Affinity purified polyclonal antibody (Catalog # ABO11716) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-PC4 Picoband Antibody ABO11716
    PC4 was detected in paraffin-embedded sections of rat liver tissues using rabbit anti- PC4 Antigen Affinity purified polyclonal antibody (Catalog # ABO11716) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-PC4 Picoband Antibody ABO11716
    PC4 was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- PC4 Antigen Affinity purified polyclonal antibody (Catalog # ABO11716) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P53999
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Activated RNA polymerase II transcriptional coactivator p15(SUB1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 10923
Other Names Activated RNA polymerase II transcriptional coactivator p15, Positive cofactor 4, PC4, SUB1 homolog, p14, SUB1, PC4, RPO2TC1
Calculated MW 14395 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human, Rat
Subcellular Localization Nucleus.
Protein Name Activated RNA polymerase II transcriptional coactivator p15
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human PC4 (96-127aa MKPGRKGISLNPEQWSQLKEQISDIDDAVRKL), different from the related mouse and rat sequences by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name SUB1
Synonyms PC4, RPO2TC1
Function General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
Cellular Location Nucleus.
Citations (0)
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Background

Activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4) or SUB1 homolog, is a protein that in humans is encoded by the SUB1 gene. This gene is mapped to 5p13.3. The transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA)-binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein. The recombinant PC4 is shown to function identically to the native protein through its interaction with TAFs.

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$ 370.00
Cat# ABO11716
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