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Anti-NFIA Picoband Antibody

     
  • WB - Anti-NFIA Picoband Antibody ABO11698
    Western blot analysis of NFIA expression in JURKAT whole cell lysates (lane 1) and COLO320 whole cell lysates (lane 2). NFIA at 69KD was detected using rabbit anti- NFIA Antigen Affinity purified polyclonal antibody (Catalog # ABO11698) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
    detail
  • IHC - Anti-NFIA Picoband Antibody ABO11698
    NFIA was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti- NFIA Antigen Affinity purified polyclonal antibody (Catalog # ABO11698) at 1 ??g/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-NFIA Picoband Antibody ABO11698
    NFIA was detected in paraffin-embedded sections of rat liver tissues using rabbit anti- NFIA Antigen Affinity purified polyclonal antibody (Catalog # ABO11698) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-NFIA Picoband Antibody ABO11698
    NFIA was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- NFIA Antigen Affinity purified polyclonal antibody (Catalog # ABO11698) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q12857
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Nuclear factor 1 A-type(NFIA) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 4774
Other Names Nuclear factor 1 A-type, NF1-A, Nuclear factor 1/A, CCAAT-box-binding transcription factor, CTF, Nuclear factor I/A, NF-I/A, NFI-A, TGGCA-binding protein, NFIA, KIAA1439
Calculated MW 55944 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Nucleus.
Protein Name Nuclear factor 1 A-type
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human NFIA (180-224aa AYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSG VFS), different from the related mouse sequence by one amino acid, and identical to the related rat sequence.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name NFIA
Synonyms KIAA1439
Function Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Cellular Location Nucleus.
Research Areas
Citations (0)
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Background

Nuclear factor 1 A-type is a protein that in humans is encoded by the NFIA gene. Nuclear factor I (NFI) proteins constitute a family of dimericDNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.

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$ 370.00
Cat# ABO11698
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