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Anti-LDHB Picoband Antibody

     
  • WB - Anti-LDHB Picoband Antibody ABO11694
    Western blot analysis of LDHB expression in rat brain extract (lane 1), NIH3T3 whole cell lysates (lane 2) and U2OS whole cell lysates (lane 3). LDHB at 37KD was detected using rabbit anti- LDHB Antigen Affinity purified polyclonal antibody (Catalog # ABO11694) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
    detail
  • IHC - Anti-LDHB Picoband Antibody ABO11694
    LDHB was detected in paraffin-embedded sections of mouse brain tissues using rabbit anti- LDHB Antigen Affinity purified polyclonal antibody (Catalog # ABO11694) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-LDHB Picoband Antibody ABO11694
    LDHB was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- LDHB Antigen Affinity purified polyclonal antibody (Catalog # ABO11694) at 1 ??g/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-LDHB Picoband Antibody ABO11694
    LDHB was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- LDHB Antigen Affinity purified polyclonal antibody (Catalog # ABO11694) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P07195
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for L-lactate dehydrogenase B chain(LDHB) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 3945
Other Names L-lactate dehydrogenase B chain, LDH-B, 1.1.1.27, LDH heart subunit, LDH-H, Renal carcinoma antigen NY-REN-46, LDHB
Calculated MW 36638 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Cytoplasm.
Protein Name L-lactate dehydrogenase B chain
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human LDHB recombinant protein (Position: E237-L334). Human LDHB shares 98% amino acid (aa) sequence identity with both mouse and rat LDHB.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name LDHB
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Cellular Location Cytoplasm. Mitochondrion inner membrane; Peripheral membrane protein
Tissue Location Predominantly expressed in aerobic tissues such as cardiac muscle.
Research Areas
Citations (0)
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Background

The LDHB gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.

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$ 370.00
Cat# ABO11694
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