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Anti-LDHA Picoband Antibody

     
  • WB - Anti-LDHA Picoband Antibody ABO11693
    Western blot analysis of LDHA expression in rat spleen extract (lane 1), ANA-1 whole cell lysates (lane 2) and JURKAT whole cell lysates (lane 3). LDHA at 37KD was detected using rabbit anti- LDHA Antigen Affinity purified polyclonal antibody (Catalog # ABO11693) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
    detail
  • IHC - Anti-LDHA Picoband Antibody ABO11693
    LDHA was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- LDHA Antigen Affinity purified polyclonal antibody (Catalog # ABO11693) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-LDHA Picoband Antibody ABO11693
    LDHA was detected in paraffin-embedded sections of rat intestine tissues using rabbit anti- LDHA Antigen Affinity purified polyclonal antibody (Catalog # ABO11693) at 1 ??g/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-LDHA Picoband Antibody ABO11693
    LDHA was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- LDHA Antigen Affinity purified polyclonal antibody (Catalog # ABO11693) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P00338
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for L-lactate dehydrogenase A chain(LDHA) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 3939
Other Names L-lactate dehydrogenase A chain, LDH-A, 1.1.1.27, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA
Calculated MW 36689 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Cytoplasm.
Protein Name L-lactate dehydrogenase A chain
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human LDHA recombinant protein (Position: A2-R106). Human LDHA shares 94.3% amino acid (aa) sequence identity with both mouse and rat LDHA.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name LDHA (HGNC:6535)
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Cellular Location Cytoplasm.
Tissue Location Predominantly expressed in anaerobic tissues such as skeletal muscle and liver.
Research Areas
Citations (0)
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Background

Lactate dehydrogenase A, also known as LDHA, is an enzyme which in humans is encoded by the LDHA gene. The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

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$ 370.00
Cat# ABO11693
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