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Anti-ADK Picoband Antibody

     
  • WB - Anti-ADK Picoband Antibody ABO11649
    Western blot analysis of ADK expression in MCF-7 whole cell lysates (lane 1). ADK at 40KD was detected using rabbit anti- ADK Antigen Affinity purified polyclonal antibody (Catalog # ABO11649) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
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  • IHC - Anti-ADK Picoband Antibody ABO11649
    ADK was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti- ADK Antigen Affinity purified polyclonal antibody (Catalog # ABO11649) at 1 ??g/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-ADK Picoband Antibody ABO11649
    ADK was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- ADK Antigen Affinity purified polyclonal antibody (Catalog # ABO11649) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P55263
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Adenosine kinase(ADK) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 132
Other Names Adenosine kinase, AK, 2.7.1.20, Adenosine 5'-phosphotransferase, ADK
Calculated MW 40545 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Isoform 1: Nucleus .
Tissue Specificity Widely expressed. Highest level in placenta, liver, muscle and kidney.
Protein Name Adenosine kinase
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human ADK recombinant protein (Position: K165-T351). Human ADK shares 88.8% and 88.2% amino acid (aa) sequence identity with mouse and rat ADK, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name ADK (HGNC:257)
Function Catalyzes the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Cellular Location [Isoform 1]: Nucleus
Tissue Location Widely expressed. Highest level in placenta, liver, muscle and kidney.
Research Areas
Citations (0)
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Background

This gene is an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene.

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$ 370.00
Cat# ABO11649
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