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Anti-Alpha Internexin Picoband Antibody

     
  • WB - Anti-Alpha Internexin Picoband Antibody ABO11621
    Western blot analysis of Alpha Internexin expression in rat brain extract (lane 1), mouse brain extract (lane 2) and 22RV1 whole cell lysates (lane 3). Alpha Internexin at 66KD was detected using rabbit anti- Alpha Internexin Antigen Affinity purified polyclonal antibody (Catalog # ABO11621) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
    detail
  • IHC - Anti-Alpha Internexin Picoband Antibody ABO11621
    Alpha Internexin was detected in paraffin-embedded sections of mouse brain tissues using rabbit anti- Alpha Internexin Antigen Affinity purified polyclonal antibody (Catalog # ABO11621) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-Alpha Internexin Picoband Antibody ABO11621
    Alpha Internexin was detected in paraffin-embedded sections of rat brain tissues using rabbit anti- Alpha Internexin Antigen Affinity purified polyclonal antibody (Catalog # ABO11621) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-Alpha Internexin Picoband Antibody ABO11621
    Alpha Internexin was detected in paraffin-embedded sections of human glioma tissues using rabbit anti- Alpha Internexin Antigen Affinity purified polyclonal antibody (Catalog # ABO11621) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q16352
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Alpha-internexin(INA) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 9118
Other Names Alpha-internexin, Alpha-Inx, 66 kDa neurofilament protein, NF-66, Neurofilament-66, Neurofilament 5, INA, NEF5
Calculated MW 55391 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Tissue Specificity Found predominantly in adult CNS.
Protein Name Alpha-internexin
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human Alpha Internexin recombinant protein (Position: A71-R161). Human Alpha Internexin shares 98.9% amino acid (aa) sequence identity with both mouse and rat Alpha Internexin.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name INA
Synonyms NEF5
Function Class-IV neuronal intermediate filament that is able to self- assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges. May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks (By similarity).
Tissue Location Found predominantly in adult CNS.
Research Areas
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Background

Alpha-Internexin (INA; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. The protein was originally purified from rat optic nerve and spinal cord. And the protein copurifies with other neurofilament subunits, as it was originally discovered, however in some mature neurons it can be the only neurofilament expressed. The protein is present in developing neuroblasts and in the Central Nervous System of adults. Meanwhile, the protein is a major component of the intermediate filament network in small interneurons and cerebellar granule cells, where it is present in the parallel fibers.

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$ 370.00
Cat# ABO11621
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