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Anti-NKCC1 Antibody
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- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IHC-F |
|---|---|
| Primary Accession | P55011 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Solute carrier family 12 member 2(SLC12A2) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 6558 |
|---|---|
| Other Names | Solute carrier family 12 member 2, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, SLC12A2, NKCC1 |
| Calculated MW | 131447 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, Mouse, By Heat Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Rat, Human, Mouse Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat |
| Subcellular Localization | Membrane; Multi-pass membrane protein. |
| Tissue Specificity | Expressed in many tissues. |
| Protein Name | Solute carrier family 12 member 2 |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human NKCC1(223-241aa RIDHYRHTAAQLGEKLLRP), identical to the related mouse and rat sequences. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Name | SLC12A2 |
|---|---|
| Function | Cation-chloride cotransporter which mediates the electroneutral transport of chloride, potassium and/or sodium ions across the membrane (PubMed:16669787, PubMed:32081947, PubMed:32294086, PubMed:33597714, PubMed:35585053, PubMed:36239040, PubMed:36306358, PubMed:7629105). Plays a vital role in the regulation of ionic balance and cell volume (PubMed:16669787, PubMed:32081947, PubMed:32294086, PubMed:7629105). |
| Cellular Location | Basolateral cell membrane; Multi-pass membrane protein |
| Tissue Location | Expressed in many tissues. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Solute carrier family 12(sodium/potassium/chloride transporters), member 2, also known as NKCC1, is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.
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