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Anti-PGK1 Antibody

     
  • WB - Anti-PGK1 Antibody ABO11352
    Anti-PGK1 antibody, ABO11352, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Lung Tissue LysateLane 4: A431 Cell LysateLane 5: COLO320 Cell LysateLane 6: HELA Cell LysateLane 7: A549 Cell LysateLane 8: JURKAT Cell Lysate
    detail
  • IHC - Anti-PGK1 Antibody ABO11352
    Anti-PGK1 antibody, ABO11352, IHC(P)IHC(P): Human Lung Cancer Tissue
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P00558
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Phosphoglycerate kinase 1(PGK1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 5230
Other Names Phosphoglycerate kinase 1, 2.7.2.3, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA
Calculated MW 44615 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Cytoplasm.
Protein Name Phosphoglycerate kinase 1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human PGK1(166-180aa FGTAHRAHSSMVGVN), identical to the related rat and mouse sequences.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the phosphoglycerate kinase family.
Protein Information
Name PGK1
Synonyms PGKA
Function Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3- diphosphoglycerate to 3-phosphoglycerate (PubMed:30323285, PubMed:7391028). In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).
Cellular Location Cytoplasm.
Tissue Location Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men
Research Areas
Citations (0)
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Background

PGK1 (Phosphoglycerate Kinase 1), also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. And the encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.

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$ 370.00
Cat# ABO11352
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