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Anti-DCI Antibody

     
  • WB - Anti-DCI Antibody ABO11072
    Anti-DCI antibody, ABO11072, Western blottingAll lanes: Anti DCI (ABO11072) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: SMMC Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 33KDObserved bind size: 33KD
    detail
  • IHC - Anti-DCI Antibody ABO11072
    Anti-DCI antibody, ABO11072, IHC(P)IHC(P): Human Mammary Cancer Tissue
    detail
  • ICC - Anti-DCI Antibody ABO11072
    Anti-DCI antibody, ABO11072, ICCICC: HELA Cell
    detail
  • ICC - Anti-DCI Antibody ABO11072
    Anti-DCI antibody, ABO11072, ICCICC: A549 Cell
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC
Primary Accession P42126
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Enoyl-CoA delta isomerase 1, mitochondrial(ECI1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 1632
Other Names Enoyl-CoA delta isomerase 1, mitochondrial, 5.3.3.8, 3, 2-trans-enoyl-CoA isomerase, Delta(3), Delta(2)-enoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, ECI1, DCI
Calculated MW 32816 MW KDa
Application Details Immunocytochemistry , 0.5-1 µg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Mitochondrion matrix.
Protein Name Enoyl-CoA delta isomerase 1, mitochondrial
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human DCI(272-290aa ADVQNFVSFISKDSIQKSL), different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name ECI1
Synonyms DCI
Function Key enzyme of fatty acid beta-oxidation (Probable). Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2- trans (2E) form in a range of enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs (By similarity) (PubMed:7818490). The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length (By similarity).
Cellular Location Mitochondrion matrix {ECO:0000250|UniProtKB:P23965}
Tissue Location Expressed in liver (at protein level).
Research Areas
Citations (0)
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Background

ECI1/DCI(Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon(position 3) to trans double bonds at beta-carbon(position 2). It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.

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$ 370.00
Cat# ABO11072
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