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Anti-DCI Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, ICC |
|---|---|
| Primary Accession | P42126 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Enoyl-CoA delta isomerase 1, mitochondrial(ECI1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 1632 |
|---|---|
| Other Names | Enoyl-CoA delta isomerase 1, mitochondrial, 5.3.3.8, 3, 2-trans-enoyl-CoA isomerase, Delta(3), Delta(2)-enoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, ECI1, DCI |
| Calculated MW | 32816 MW KDa |
| Application Details | Immunocytochemistry , 0.5-1 µg/ml, Human, - Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
| Subcellular Localization | Mitochondrion matrix. |
| Protein Name | Enoyl-CoA delta isomerase 1, mitochondrial |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human DCI(272-290aa ADVQNFVSFISKDSIQKSL), different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Name | ECI1 |
|---|---|
| Synonyms | DCI |
| Function | Key enzyme of fatty acid beta-oxidation (Probable). Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2- trans (2E) form in a range of enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs (By similarity) (PubMed:7818490). The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length (By similarity). |
| Cellular Location | Mitochondrion matrix {ECO:0000250|UniProtKB:P23965} |
| Tissue Location | Expressed in liver (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
ECI1/DCI(Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon(position 3) to trans double bonds at beta-carbon(position 2). It plays a particularly important role in the metabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of enzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray crystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold composed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA Delta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid degradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so by shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or trans-enoyl-CoA to 2-trans-enoyl-CoA.
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