Anti-DUSP3 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB |
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Primary Accession | P51452 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Dual specificity protein phosphatase 3(DUSP3) detection. Tested with WB in Human;Mouse;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 1845 |
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Other Names | Dual specificity protein phosphatase 3, 3.1.3.16, 3.1.3.48, Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase, VHR, DUSP3, VHR |
Calculated MW | 20478 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
Subcellular Localization | Nucleus . |
Protein Name | Dual specificity protein phosphatase 3 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human DUSP3(155-171aa RQNREIGPNDGFLAQLC), identical to the related rat and mouse sequences. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. |
Name | DUSP3 |
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Synonyms | VHR |
Function | Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines (PubMed:10224087, PubMed:11863439). Specifically dephosphorylates and inactivates ERK1 and ERK2 (PubMed:10224087, PubMed:11863439). |
Cellular Location | Nucleus. Cytoplasm, cytoskeleton, flagellum axoneme {ECO:0000250|UniProtKB:Q9D7X3} |

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Background
DUSP3(Dual-specificity phosphatase 3), also called VHR, is a member of the dual specificity protein phosphatase subfamily. DUSPs constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. DUSP3 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP(mitogen-activated protein kinase phosphatase) class of DUSPs. The DUSP3 gene is mapped on 17q21.31. Confocal microscopy demonstrated that phosphorylated VHR accumulated at the immune synapse between the T cell and the antigen-presenting cell in the presence of antigen. Tyrosine phosphorylation of VHR affects protein-protein interaction, subcellular location, or substrate targeting, given that tyr138 is located on the opposite side of the VHR catalytic center.

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