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Anti-IL5RA Antibody
- SPECIFICATION
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- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q01344 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Interleukin-5 receptor subunit alpha(IL5RA) detection. Tested with WB in Human. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 3568 |
|---|---|
| Other Names | Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125, CD125, IL5RA, IL5R |
| Calculated MW | 47685 MW KDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Tissue Specificity | Expressed on eosinophils and basophils. |
| Protein Name | Interleukin-5 receptor subunit alpha(IL-5 receptor subunit alpha/IL-5R subunit alpha/IL-5R-alpha/IL-5RA) |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human IL5RA(53-68aa NPDQEQRNVNLEYQVK). |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the type I cytokine receptor family. Type 5 subfamily. |
| Name | IL5RA |
|---|---|
| Synonyms | IL5R |
| Function | Cell surface receptor that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:9378992). Acts by forming a heterodimeric receptor with CSF2RB subunit and subsequently binding to interleukin-5 (PubMed:1495999, PubMed:22528658). In unstimulated conditions, interacts constitutively with JAK2. Heterodimeric receptor activation leads to JAK2 stimulation and subsequent activation of the JAK-STAT pathway (PubMed:9516124). |
| Cellular Location | Membrane; Single-pass type I membrane protein. |
| Tissue Location | Expressed on eosinophils and basophils. |
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Provided below are standard protocols that you may find useful for product applications.
Background
IL5RA(INTERLEUKIN 5 RECEPTOR, ALPHA), also known as CD125, is a subunit of the Interleukin-5 receptor. IL5RA also denotes its human gene. The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor which is composed of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3(IL3), colony stimulating factor 2(CSF2/GM-CSF), and interleukin 5(IL5). The IL5RA gene is mapped to 3p26-p24 by Southern blot analysis of DNA from a panel of mouse-human hybrid somatic cell lines complemented by in situ hybridization. And its Cytogenetic location is 3p26.2. Within this 15-residue stretch of IL5RA, the C-terminal phenylalanine is critical. The binding of this protein to IL5 depends on the beta subunit which is activated by the ligand binding and is required for the biological activities of IL5.
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