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Anti-BCAT1 Antibody
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- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | P54687 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1) detection. Tested with WB, IHC-P in Human. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 586 |
|---|---|
| Other Names | Branched-chain-amino-acid aminotransferase, cytosolic, BCAT(c), 2.6.1.42, Protein ECA39, BCAT1, BCT1, ECA39 |
| Calculated MW | 42966 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat Western blot, 0.1-0.5 µg/ml, Human |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma. |
| Protein Name | Branched-chain-amino-acid aminotransferase, cytosolic(BCAT(c)) |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT1(360-375aa KLASRILSKLTDIQYGR), different from the related mouse sequence by one amino acid, rat sequence by three amino acids. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Name | BCAT1 |
|---|---|
| Synonyms | BCT1, ECA39 {ECO:0000303|PubMed:8692959} |
| Function | Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:P54690}. |
| Tissue Location | During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma |
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Provided below are standard protocols that you may find useful for product applications.
Background
BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.
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