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Anti-BCAT2 Antibody

     
  • WB - Anti-BCAT2 Antibody ABO10820
    Anti-BCAT2 antibody, ABO10820, Western blottingAll lanes: Anti BCAT2 (ABO10820) at 0.5ug/mlLane 1: A431 Whole Cell Lysate at 40ugLane 2: SW620 Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: CEM Whole Cell Lysate at 40ugPredicted bind size: 41KDObserved bind size: 41KD
    detail
  • IHC - Anti-BCAT2 Antibody ABO10820
    Anti-BCAT2 antibody, ABO10820, IHC(P)IHC(P): Human Colon Cancer Tissue
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession O15382
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT2) detection. Tested with WB, IHC-P in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 587
Other Names Branched-chain-amino-acid aminotransferase, mitochondrial, BCAT(m), 2.6.1.42, Placental protein 18, PP18, BCAT2, BCATM, BCT2, ECA40
Calculated MW 44288 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat
Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Isoform A: Mitochondrion.
Tissue Specificity Ubiquitous.
Protein Name Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT(m))
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT2(377-392aa KEIQYGIRAHEWMFPV).
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
Protein Information
Name BCAT2
Function Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine (PubMed:17050531, PubMed:25653144, PubMed:8702755). May also function as a transporter of branched chain alpha-keto acids (By similarity).
Cellular Location Mitochondrion {ECO:0000250|UniProtKB:O35854}.
Tissue Location Ubiquitous..
Research Areas
Citations (0)
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Background

BCAT2, branched-chain aminotransferase 2, encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. The human BCAT2 gene is mapped to 19q13.

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$ 370.00
Cat# ABO10820
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