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Anti-GRP94 Antibody

     
  • WB - Anti-GRP94 Antibody ABO10665
    Anti-GRP94 antibody, ABO10665, Western blottingLane 1: RAJI Cell LysateLane 2: MCF-7 Cell Lysate
    detail
  • IHC - Anti-GRP94 Antibody ABO10665
    Anti-GRP94 antibody, ABO10665, IHC(P)IHC(P): HELA Cell
    detail
  • IHC - Anti-GRP94 Antibody ABO10665
    Anti-GRP94 antibody, ABO10665, IHC(P)IHC(P): Human Lung Cancer Tissue
    detail
  • IHC - Anti-GRP94 Antibody ABO10665
    Anti-GRP94 antibody, ABO10665, IHC(P)IHC(P): Rat Brain Tissue
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, ICC
Primary Accession P14625
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Endoplasmin(HSP90B1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 7184
Other Names Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, HSP90B1, GRP94, TRA1
Calculated MW 92469 MW KDa
Application Details Immunocytochemistry , 0.5-1 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Protein Name Endoplasmin
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human GRP94(687-700aa INPRHPLIRDMLRR), identical to the related mouse and rat sequences.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name HSP90B1 (HGNC:12028)
Function Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum- Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059).
Cellular Location Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen {ECO:0000250|UniProtKB:P41148}. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Research Areas
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Background

Heat shock protein 90kDa beta member 1 (HSP90B1), known also as endoplasmin, GRP94, is a chaperone protein that in humans is encoded by the HSP90B1 gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation.

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$ 370.00
Cat# ABO10665
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