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Anti-CD34 Antibody

     
  • WB - Anti-CD34 Antibody ABO10660
    Anti-CD34 antibody, ABO10660, Western blottingLane 1: Recombinant Human CD34 Protein 10ngLane 2: Recombinant Human CD34 Protein 5ngLane 3: Recombinant Human CD34 Protein 2.5ng
    detail
  • IHC - Anti-CD34 Antibody ABO10660
    Anti-CD34 antibody, ABO10660, IHC(P)IHC(P): Rat Kidney Tissue
    detail
  • IHC - Anti-CD34 Antibody ABO10660
    Anti-CD34 antibody, ABO10660, IHC(P)IHC(P): Human Lung Cancer Tissue
    detail
  • IHC - Anti-CD34 Antibody ABO10660
    Anti-CD34 antibody, ABO10660, IHC(F)IHC(F): Rat Kidney Tissue
    detail
  • IHC - Anti-CD34 Antibody ABO10660
    Anti-CD34 antibody, ABO10660, IHC(F)IHC(F): Rat Placenta Tissue
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F
Primary Accession P28906
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Hematopoietic progenitor cell antigen CD34(CD34) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 947
Other Names Hematopoietic progenitor cell antigen CD34, CD34, CD34
Calculated MW 40716 MW KDa
Application Details Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Mouse, Rat, Human
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Membrane; Single-pass type I membrane protein.
Tissue Specificity Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Protein Name Hematopoietic progenitor cell antigen CD34
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human CD34(366-382aa QATSRNGHSARQHVVAD), identical to the related mouse and rat sequences.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the CD34 family.
Protein Information
Name CD34
Function Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Cellular Location Membrane; Single-pass type I membrane protein.
Tissue Location Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues
Research Areas
Citations (0)
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Background

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

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$ 370.00
Cat# ABO10660
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