Anti-CD34 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IHC-F |
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Primary Accession | P28906 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Hematopoietic progenitor cell antigen CD34(CD34) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 947 |
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Other Names | Hematopoietic progenitor cell antigen CD34, CD34, CD34 |
Calculated MW | 40716 MW KDa |
Application Details | Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Mouse, Rat, Human Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat |
Subcellular Localization | Membrane; Single-pass type I membrane protein. |
Tissue Specificity | Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. |
Protein Name | Hematopoietic progenitor cell antigen CD34 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CD34(366-382aa QATSRNGHSARQHVVAD), identical to the related mouse and rat sequences. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Belongs to the CD34 family. |
Name | CD34 |
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Function | Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. |
Cellular Location | Membrane; Single-pass type I membrane protein. |
Tissue Location | Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.
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