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Anti-XAF1 Antibody
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- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | Q6GPH4 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for XIAP-associated factor 1(XAF1) detection. Tested with WB, IHC-P in Human. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 54739 |
|---|---|
| Other Names | XIAP-associated factor 1, BIRC4-binding protein, XAF1, BIRC4BP, XIAPAF1 |
| Calculated MW | 34626 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat Western blot, 0.1-0.5 µg/ml, Human |
| Subcellular Localization | Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment. |
| Tissue Specificity | Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level). . |
| Protein Name | XIAP-associated factor 1 |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human XAF1(283-301aa QEKCRWLASSKGKQVRNFS). |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Contains 1 TRAF-type zinc finger. |
| Name | XAF1 |
|---|---|
| Synonyms | BIRC4BP, XIAPAF1 |
| Function | Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondria, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. |
| Cellular Location | Cytoplasm. Nucleus. Mitochondrion. Note=Found in the cytoplasm and nucleus of placental syncytiotrophoblasts Translocates to mitochondria upon TNF-alpha treatment [Isoform 5]: Nucleus. |
| Tissue Location | Widely expressed. Expression is frequently down- regulated in cancer cell lines. Isoform 5 is widely expressed Expressed in placenta (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
XIAP associated factor-1, also known as XAF1, is a human gene. X-linked inhibitor of apoptosis(XIAP; MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP(BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. And alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.
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