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Anti-FGF8 Antibody
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | P55075 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Fibroblast growth factor 8(FGF8) detection. Tested with WB, IHC-P in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 2253 |
|---|---|
| Other Names | Fibroblast growth factor 8, FGF-8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8, FGF8, AIGF |
| Calculated MW | 26525 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, Mouse, By Heat Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
| Subcellular Localization | Secreted. |
| Protein Name | Fibroblast growth factor 8(FGF-8) |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human FGF8(163-185aa FMKRLPRGHHTTEQSLRFEFLNY), identical to the related rat and mouse sequences. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the heparin-binding growth factors family. |
| Name | FGF8 |
|---|---|
| Synonyms | AIGF |
| Function | Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system (PubMed:16384934, PubMed:16597617, PubMed:8663044). Plays a role in neurite outgrowth in hippocampal cells (PubMed:21576111). |
| Cellular Location | Secreted. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Fibroblast growth factor 8(androgen-induced), also known as FGF8 or AIGF, is a human gene which maps to 10q24. The protein encoded by this gene are secreted proteins that interact with FGF tyrosine kinase receptors to mediate growth and development. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The temporal and spatial patterns of this gene expression suggest that FGF8 is involved in gastrulation, regionalization of the brain, and organogenesis of the limb and face as an embryonic epithelial factor. The adult expression of FGF8 is restricted to gonads, including testes and ovaries. FGF8 stimulated growth of human prostate carcinoma cells and mouse fibroblasts and mammary carcinoma cells in a dose-dependent manner. It also may play an important role in growth and patterning of limbs, face, and central nervous system. FGF8 is expressed in increased levels in breast cancer and in lactating human breast; it was also detected in human milk. A survey of other normal tissues showed that FGF8 is expressed in the proliferative cells of the skin and epithelial cells in colon, ovary, fallopian tube, and uterus. FGF8 in adults expression is restricted to the gonads. We detected rat ovary in our lab.
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