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Anti-GST3/GST Pi Antibody

     
  • WB - Anti-GST3/GST Pi Antibody ABO10527
    Anti-GST3/GST pi antibody, ABO10527, Western blottingLane 1: MCF-7 Cell LysateLane 2: COLO320 Cell Lysate
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P09211
Host Rabbit
Reactivity Human, Mouse
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Glutathione S-transferase P(GSTP1) detection. Tested with WB in Human;Mouse.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 2950
Other Names Glutathione S-transferase P, 2.5.1.18, GST class-pi, GSTP1-1, GSTP1, FAEES3, GST3
Calculated MW 23356 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human, Mouse
Subcellular Localization Cytoplasm . Mitochondrion . Nucleus . The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization.
Protein Name Glutathione S-transferase P
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human GST3(197-210aa PEYVNLPINGNGKQ), different from the related mouse sequence by two amino acids.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the GST superfamily. Pi family.
Protein Information
Name GSTP1 (HGNC:4638)
Synonyms FAEES3, GST3
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
Cellular Location Cytoplasm. Mitochondrion. Nucleus. Note=The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization
Research Areas
Citations (0)
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Background

Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST(GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11 .Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.

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$ 370.00
Cat# ABO10527
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