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Anti-Collagen, Type IV Antibody (Monoclonal, COL-94)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, IHC-F |
|---|---|
| Primary Accession | P02462 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Human |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for Collagen, Type IV, collagen, type IV, alpha-1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen,type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6 (COL4A1; COL4A3; COL4A4; COL4A5; COL4A6) detection. Tested with IHC-P, IHC-F in Human. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 1282 |
|---|---|
| Other Names | Collagen alpha-1(IV) chain, Arresten, COL4A1 |
| Calculated MW | 160615 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Human |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Tissue Specificity | Highly expressed in placenta. . |
| Protein Name | Collagen alpha-1(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | COL-94 |
| Immunogen | Human collagen type IV. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the type IV collagen family. |
| Name | COL4A1 (HGNC:2202) |
|---|---|
| Function | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. |
| Cellular Location | Secreted, extracellular space, extracellular matrix, basement membrane {ECO:0000250|UniProtKB:P02463} |
| Tissue Location | Highly expressed in placenta. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Collagen IV is comprised of a third chain(alpha-3) together with the 2 classical ones, alpha-1 and alpha-2. COL4A3 gene is localized to 2q36-q37 by analysis of somatic cell hybrids and by in situ hybridization. The alpha-3 chain of type IV collagen induces autoimmune Goodpasture syndrome. Mutations in the COL4A4 and COL4A3 genes cause familial benign hematuria.
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