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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100)   

Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100)

     
  • WB - Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100) ABO10400
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  • IHC - Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100) ABO10400
    Anti-Bcl-2 antibody (monoclonal), ABO10400, IHC(P)IHC(P): Human Intestinal Cancer Tissue
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  • IHC - Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100) ABO10400
    Anti-Bcl-2 antibody (monoclonal), ABO10400, IHC(P)IHC(P): Human Mammary Cancer Tissue
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, ICC
Primary Accession P49950
Host Mouse
Isotype Mouse IgG1
Reactivity Human
Clonality Monoclonal
Format Lyophilized
Description Mouse IgG monoclonal antibody for Bcl-2, B-cell CLL/lymphoma 2 (BCL2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Additional Information
Gene ID 24224
Other Names Apoptosis regulator Bcl-2, Bcl2, Bcl-2
Calculated MW 26622 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.4-1 µg/ml, Human, By Heat

Immunocytochemistry , 1 µg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.4-1 µg/ml, Human, -
Western blot, 1-2 µg/ml, Human
Subcellular Localization Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
Tissue Specificity Expressed in a variety of tissues, with highest levels in reproductive tissues. In the adult brain, expression is localized in mitral cells of the olfactory bulb, granule and pyramidal neurons of hippocampus, pontine nuclei, cerebellar granule neurons, and in ependymal cells. In prenatal brain, expression is higher and localized in the neuroepithelium and in the cortical plate.
Protein Name Apoptosis regulator Bcl-2
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clone Names Bcl-2-100
Immunogen Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.
Purification Ascites
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the Bcl-2 family.
Protein Information
Name Bcl2
Synonyms Bcl-2
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function. May attenuate inflammation by impairing NLRP1- inflammasome activation, hence CASP1 activation and IL1B release.
Cellular Location Mitochondrion outer membrane {ECO:0000250|UniProtKB:P10415}; Single-pass membrane protein. Nucleus membrane {ECO:0000250|UniProtKB:P10415}; Single- pass membrane protein. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P10415}; Single-pass membrane protein. Cytoplasm {ECO:0000250|UniProtKB:P10417}
Tissue Location Expressed in a variety of tissues, with highest levels in reproductive tissues. In the adult brain, expression is localized in mitral cells of the olfactory bulb, granule and pyramidal neurons of hippocampus, pontine nuclei, cerebellar granule neurons, and in ependymal cells. In prenatal brain, expression is higher and localized in the neuroepithelium and in the cortical plate
Research Areas
Citations (0)
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Background

Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

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$ 370.00
Cat# ABO10400
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