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> home > Products > Primary Antibodies > Antibody Collections > Antibody Pairs > Anti-Human VEGF Antibody
Anti-Human VEGF Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, E |
|---|---|
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Preparation | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hVEGF (human VEGF). Anti-Human VEGF specific antibody was purified by affinity chromatography employing immobilized hVEGF matrix. |
|---|---|
| WesternBlot | To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Sandwich | To detect hVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human VEGF (60-342BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF. |
| Immunohistochemistry | This antibody stained formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended concentrations are 2.5 - 5.5 mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. |
| Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of hVEGF (10 ng/ml), a concentration of 0.05 - 0.1 µg/ml of this antibody is required. |
| Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Storage | -20°C |
| Precautions | Anti-Human VEGF Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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$ 275.00
$ 216.00
Cat# ABG10576-U100
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