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Anti-Human TNF-β Antibody
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Application 
| WB, IHC, E |
|---|---|
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Preparation | Produced from sera of rabbits pre-immunized with highly pure recombinant Human TNF-β. Anti-Human TNF-β specific antibody was purified by affinity chromatography employing immobilized Human TNF-β matrix. |
|---|---|
| WesternBlot | To detect Human TNF-β by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-β is 1.5-3.0 ng/lane, under either reducing non-reducing conditions. |
| Sandwich | To detect Human TNF-β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human TNF-β (60-325BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-β. |
| Immunohistochemistry | This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant carcinoma. The recommended concentration is 0.50 µg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. |
| Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-β (0.5 ng/ml), a concentration of < 0.1 μg/ml of this antibody is required. |
| Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Storage | -20°C |
| Precautions | Anti-Human TNF-β Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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$ 275.00
$ 216.00
Cat# ABG10556-U100
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