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> home > Products > Primary Antibodies > Antibody Collections > Antibody Pairs > Anti-Human PAI-1 Antibody
Anti-Human PAI-1 Antibody
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- BACKGROUND
Application 
| WB, IHC, E |
|---|---|
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Preparation | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPAI-1 (human Plasminogen Activator Inhibitor-1). Anti-Human PAI-1 specific antibody was purified by affinity chromatography employing immobilized hPAI-1 matrix. |
|---|---|
| WesternBlot | To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Sandwich | To detect hPAI-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human PAI-1 (60-263BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPAI-1. |
| Immunohistochemistry | This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 62.5 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information. |
| Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of hPAI-1 (4.5 μg/ml), a concentration of at least 15.0 μg/ml of this antibody is required. |
| Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Storage | -20°C |
| Precautions | Anti-Human PAI-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Research Areas
Citations (0)
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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If you have any additional inquiries please email technical services at tech@abcepta.com.
$ 275.00
$ 216.00
Cat# ABG10453-U100
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