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Anti-Human MIP-3β Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Reactivity Human
Host Mouse
Clonality Monoclonal
Additional Information
Preparation Produced in BALB/c mice using highly pure recombinant Human MIP-3β as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
WesternBlot To detect Human MIP-3β by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3β is 2.0-4.0 ng/lane, under reducing conditions.
Sandwich In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect recombinant Human MIP-3β when used with BioGems' biotinylated antigen affinity purified anti-Human MIP-3β (60-228BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
Immunohistochemistry This antibody stained PBMC.  The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody.  Optimal concentrations and conditions may vary.  Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. 
Formulation A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage-20°C
PrecautionsAnti-Human MIP-3β Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
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$ 216.00
Cat# ABG10420-U500
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