Pink1 | Park2 | Mitophagy | E3 ligase Parkin

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18,000+ products cover the ten top research categories, including cancer, metabolism, cardiovascular, neuroscience, and stem cells. Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. Quality is embedded in the production process to assure the best antibody for you.

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Abgent develops 1000+ new products per year. We provide tools for path-breaking research by developing antibodies that detect a comprehensive library of novel and established targets. For established targets we seek to add antibodies that recognize new epitopes, including post-translational modifications such as phosphorylation and methylation. For new targets we consult with leading experts to accelerate development of antibodies that will propel state-of-the-art research in cellular health and disease.

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Research Focus

Greneral Collections

Peptides

SARS Peptides

Individual peptides for SARS-CoV-2 Spike glycoprotein. In order to study the specificity of cellular immune responses against SARS CoV-2 and potential immunity caused by other human Corona Viruses, Abcepta provides Spike peptide individually, as pools and in plate. These peptides can be used for antigen specific T-cell stimulation in T-cell assays or T-cell expansion.

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Synthetic Peptides

All peptides are manufactured in the San Diego, California. Custom peptide services include long peptides (>100 aa), cyclic peptides, difficult sequences, fluorescent labels, phospho-peptides and other post-translational modifications.

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The majority of Abcepta antibodies are produced using peptide immunogens. These immuno-specific peptides can be used as blocking agents when using the complementary antibodies in a range of applications.

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Cell/Tissues/Lysates

Abcepta's portfolio of cell lines, tissues and lysates are drawn from a range of species and immortalized cell lines. All our cell lines and lysates are validated in key applications.

These tissue lysates can be used in applications such as SDS-PAGE and Western blotting. Whole cell lysates can be used as positive controls for applications such as ELISAs, immunoprecipitation (IP) and Western blotting. Obtaining high-quality whole cell lysates can be tedious as well as time consuming. Abcepta offers a comprehensive portfolio of whole cell lysates for research use. Browse our catalog to find the right whole cell lysate for your experiment.

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Citations

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

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	Parkin
Introduction
Damaged mitochondria can be degraded through a type of organelle-specific autophagy called mitophagy. The Ser/ Thr kinase PTEN-induced putative kinase 1 (PINK1) and the ubiquitin E3 ligase Parkin (Parkinson juvenile disease protein 2, PARK2) are known to promote mitophagy. Parkin ubiquitylates mitochondrial proteins and causes mitochondria to become engulfed by isolation membranes that then fuse with lysosomes (figure bellow). In this model, the autophagy adaptor protein p62/ SQSTM1 (p62) binds to ubiquitinated proteins via its ubiquitinassociated domain and to LC3 on the phagophore via its LC3- interacting region. Thus, the binding of p62 to ubiquitinated mitochondrial proteins tethers the mitochondrion to the LC3- positive phagophore for engulfment. PARKIN also interacts with AMBRA1 (Activating molecule in Beclin-1-regulated autophagy protein 1) at mitochondria to promote mitochondrial clearance, and depolarization of mitochondria increases the interaction between Parkin and AMBRA1.	Fluorescent confocal image of NCI-H460 cells stained with Parkin Antibody (N-term)(Cat#AP6402a). NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min) and incubated with Parkin primary antibody. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).Nuclei were counterstained with DAPI.

Recombinant Antibody

Parkin