Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
COVID19
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Loading Control   >   GAPDH Antibody   

GAPDH Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - GAPDH Antibody AM1020b
    All lanes: Anti-GAPDH Antibody at 1:1000 dilution + C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated (ASP1613) at 1/15000 dilution. Observed band size: 36KDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IF - GAPDH Antibody AM1020b
    Fluorescent image of Hela cells stained with XAF1 GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
    detail
  • WB - GAPDH Antibody AM1020b
    All lanes : Anti-GAPDH Antibody at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: C6 whole cell lysates Lane 3: Hela whole cell lysates Lane 4: HUVEC whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - GAPDH Antibody AM1020b
    All lanes : Anti-GAPDH Antibody at 1:8000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lane 3: COS-7 whole cell lysates Lane 4: mouse brain lysates Lane 5: C6 whole cell lysates Lane 6: NIH/3T3 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - GAPDH Antibody AM1020b
    Western blot analysis of anti-GAPDH Monoclonal Antibody (Cat. #AM1020b) in CEM cell line lysates (35μg/lane). GAPDH(arrow) was detected using the purified Mab.
    detail
  • IHC-P - GAPDH Antibody AM1020b
    Immunohistochemical analysis of paraffin-embedded H.kidney section using GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC-P - GAPDH Antibody AM1020b
    Immunohistochemical analysis of paraffin-embedded H.stomach section using GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • SPECIFICATION
  • CITATIONS: 44
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P04406
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone/Animal Names 1A10A10
Calculated MW 36053 Da
Additional Information
Gene ID 2597
Other Names Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, GAPDH, GAPD
Target/Specificity GAPDH recombinant protein is used to produce this monoclonal antibody.
Dilution WB~~1:2000~10000
IF~~1:25
IHC-P~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGAPDH Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GAPDH {ECO:0000303|PubMed:2987855, ECO:0000312|HGNC:HGNC:4141}
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D- glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1- dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Cellular Location Cytoplasm, cytosol. Nucleus {ECO:0000250|UniProtKB:P04797}. Cytoplasm, perinuclear region. Membrane Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P04797} Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261) {ECO:0000250|UniProtKB:P04797, ECO:0000269|PubMed:12829261}
Research Areas
Citations ( 0 )

Background

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

References

Inhibition of glyceraldehyde-3-phosphate dehydrogenase activity by antibodies present in the cerebrospinal fluid of patients with multiple sclerosis. Kölln J, et al. J Immunol, 2010 Aug 1. PMID 20610654. Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 May 14. PMID 20471030. Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of melanoma antigens using a Serological Proteome Approach (SERPA). Suzuki A, et al. Cancer Genomics Proteomics, 2010 Jan-Feb. PMID 20181627. siah-1 Protein is necessary for high glucose-induced glyceraldehyde-3-phosphate dehydrogenase nuclear accumulation and cell death in Muller cells. Yego EC, et al. J Biol Chem, 2010 Jan 29. PMID 19940145.

FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

$ 365.00
$ 140.00
Cat# AM1020b
Size:
Quantity:
Availability: In Stock
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"